48 Hour Chick Embryo Serial Cross Section

15-09-2021 admin

Diagrams of 33-, 48- and 72-hour chick embryos @Cebra-Thomas, 2003. Last Modified: 20 January 2003 Lab Protocols Students Cebra-Thomas Course Links.

On this page the following view of the embryology in chicken are displayed:

Chick 48-Hour Embryo Slide, serial c.s. Sequential cross sections of a 48-hour chick embryo mounted in rows on microscope slides. A prominent heart and a few branchial grooves are present. This stage is characterized by ventral flexion and dextral torsion, resulting in a 'C-shaped' embryo. 8 - Embryo of chicken (24 hour) w.m. 9 - Embryo of chicken (24 hour) serial transverse sections. 10 - Embryo of chicken (48 hour) w.m. 11 - Embryo of chicken (48 hour) serial transverse sections. 12 - Embryo of chicken (72 hour) w.m. 13 - Embryo of chicken (72 hour) serial transverse sections. 14 - Embryo of chicken (96 hour) w.m.

Whole mount preparation 48 hours (More...)

Cross sections 48 hours; eye and heart formation (More...)

Whole mount preparation 55 hours (More...)

Stage 48 hrs

96 Hour Chick Embryo

Whole mount preparation 48 hrs
Information: The position of the embryo with respect to the yolk changes strongly about 48 hours after fertilization. In addition to the head fold of the amnion, also the lateral and caudal amniotic folds begin to form. The outgrowth of the cranial flexure is so strong that the forebrain and hindbrain vesicles become almost located to each other. The cephalic region of the embryo is twisted in such a manner that the left side comes to lie next to the yolk. A second flexure appears at the transition of the head and the body just behind the heart region. The embryo takes now the shape of a C. The head becomes covered by a double fold. These folds definitely establish the first extra embryonic membrane (=outside of the embryo): the amnion membrane. The vitelline (yolk rich) arteries and veins become connected with the extra embryonic circulatory vessels. A few branchial grooves are already visible. The brain divides in to 5 vesicles: telencephalon and diencephalon (both formed by the division of the forebrain vesicle), mesencephalon, metencephalon and myencephalon (both formed by the division of the hindbrain vesicle). The lens placode (placode=plate) will form the lens vesicle, the optic vesicle will become the optic cup and the auditory placode the auditory pit. The heart differentiates in to 4 compartiments: the sinus venosus, connected with the veins, the atrium, the U-shaped ventricle and the bulbus cordis. The atrium and ventricle are well distinguishable in the figure.
Embryology in chicken 48 hours after fertilization: stained whole-mount preparation 1 Amnion, 2 Metencephalon, 3 Mesencephalon, 4 Optic cup + lens, 5 Prosencephalon, 6 Otic vesicle, 7 Branchial arches, 8 Atrium , 9 Ventricle, 10 Lateral fold, 11 Lateral mesoderm, 12 Vitelline arteria / vein, 13 Somite, 14 Spine, 15 Tail fold

Whole mount preparation 48 hrs

Information:
The position of the embryo with respect to the yolk changes strongly about 48 hours after fertilization. In addition to the head fold of the amnion, also the lateral and caudal amniotic folds begin to form. The outgrowth of the cranial flexure is so strong that the forebrain and hindbrain vesicles become almost located to each other. The cephalic region of the embryo is twisted in such a manner that the left side comes to lie next to the yolk. A second flexure appears at the transition of the head and the body just behind the heart region. The embryo takes now the shape of a C. The head becomes covered by a double fold. These folds definitely establish the first extra embryonic membrane (=outside of the embryo): the amnion membrane. The vitelline (yolk rich) arteries and veins become connected with the extra embryonic circulatory vessels. A few branchial grooves are already visible. The brain divides in to 5 vesicles: telencephalon and diencephalon (both formed by the division of the forebrain vesicle), mesencephalon, metencephalon and myencephalon (both formed by the division of the hindbrain vesicle). The lens placode (placode=plate) will form the lens vesicle, the optic vesicle will become the optic cup and the auditory placode the auditory pit. The heart differentiates in to 4 compartiments: the sinus venosus, connected with the veins, the atrium, the U-shaped ventricle and the bulbus cordis. The atrium and ventricle are well distinguishable in the figure.

Embryology in chicken 48 hours after fertilization: stained whole-mount preparation
1 Amnion, 2 Metencephalon, 3 Mesencephalon, 4 Optic cup + lens, 5 Prosencephalon, 6 Otic vesicle, 7 Branchial arches, 8 Atrium , 9 Ventricle, 10 Lateral fold, 11 Lateral mesoderm, 12 Vitelline arteria / vein, 13 Somite, 14 Spine, 15 Tail fold